kamniroИсследования водных биологических ресурсов Камчатки и северо-западной части Тихого океанаThe researches of the aquatic biological resources of Kamchatka and the North-West Part of the Pacific Ocean2072-82122782-6236«КамчатНИРО»10.15853/2072-8212.2023.71.23-33kamniro-241Research ArticleОРИГИНАЛЬНЫЕ НАУЧНЫЕ СТАТЬИFULL ARTICLESПопуляционно-генетическое разнообразие кижуча (Oncorhynchus kisutch Walbaum) на азиатской части ареала по результатам анализа микросателлитных локусовPopulation genetic diversity of coho salmon (Oncorhynchus kisutch Walbaum) in the Asian part of its range based on the results of microsatellite loci analysisСошнинаВ. А.SoshninaV. A.

Сошнина Валерия Александровна — гл. специалист отдела молекулярной генетики

Москва

Valeria A. Soshnina – Principal Specialist of the Molecular Genetics Department

Moscow

valnestle@yandex.ruЗеленинаД. А.ZeleninaD. A.

Зеленина Дарья Александровна — канд. биол. наук, вед. науч. сотрудник отдела молекулярной генетики

Москва

Darya A. Zelenina – Ph. D. (Biology), Leading Scientist of the Molecular Genetics Department

Moscow

d.zelenina@gmail.comВсероссийский научно-исследовательский институт рыбного хозяйства и океанографии (ВНИРО)РоссияRussian Federal Research Institute of Fisheries and Oceanography (VNIRO)Russian Federation2023060320240712333Copyright © Сошнина В.А., Зеленина Д.А., 20242024Сошнина В.А., Зеленина Д.А.Soshnina V.A., Zelenina D.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://kamniro.elpub.ru/jour/article/view/241

Впервые исследован полиморфизм 10 ядерных микросателлитных локусов в популяциях кижуча на азиатской части ареала. Выявлены три генетически обособленных региональных комплекса стад: камчатский, североохотоморский и сахалинский. Показано, что для всех популяций вне зависимости от их географического положения характерен высокий уровень генетического полиморфизма.

Polymorphism of 10 nuclear microsatellite loci in coho salmon populations in the Asian part of the range was studied for the first time. Three genetically distinct regional stock complexes were identified: Kamchatka, northern part of the Sea of Okhotsk and Sakhalin. It was shown that all populations, regardless of their geographic location, are characterized by a high level of genetic polymorphism.

кижуч (Oncorhynchus kisutch Walbaum)микросателлитыгенетическое разнообразиеполиморфизм ядерных локусовcoho salmon (Oncorhynchus kisutch Walbaum)microsatellitesgenetic diversitypolymorphism of nuclear lociавторы признательны сотрудникам ФГБНУ «ВНИРО» и его Камчатского, Магаданского и Хабаровского филиалов, принимавших участие в сборе материала на Камчатке и материковом побережье Охотского моря, а также Е.А. Кирилловой (Институт проблем экологии и эволюции им. А.Н. Северцова РАН) за сбор образцов на острове Сахалин. Работа выполнена в рамках госзадания ФГБНУ «ВНИРО» и при поддержке гранта Минобрнауки РФ (Соглашение № 075-15-2021-1084).authors are grateful to the staff of FSBSI VNIRO and its Kamchatka, Magadan and Khabarovsk branches who participated in the collection of material in Kamchatka and the mainland coast of the Sea of Okhotsk and to E.A. Kirillova (A.N. Severtsov Institute for Problems of Ecology and Evolution, Russian Academy of Sciences) for collecting samples on Sakhalin Island. The work was carried out under the state assignment of FSBSI VNIRO and supported by a grant from the Ministry of Education and Science of the Russian Federation (Agreement No. 075-15-2021-1084).ReferencesЗеленина Д.А., Сошнина В.А., Сергеев А.А. 2020. Филогеография и митохондриальный полиморфизм кижуча азиатских стад // Молекулярная биология. Т. 54. С. 997–1005.Zelenina D.A., Soshnina V.A., Sergeev A.A. Phylogeography and Mitochondrial Polymorphism of Asian Coho Salmon. Molecular biology, 2020, vol. 54, № 6, pp. 997–1005.Зорбиди Ж.Х. 2010. Кижуч азиатских стад. Петропавловск-Камчатский: КамчатНИРО. 206 с.Zorbidi Z.K. Kizhuch aziatskih stad [Asian coho salmon stocks]. Petropavlovsk-Kamchatsky: KamchatNIRO, 2010, 206 p.Пустовойт С.П. 1995. Особенности генетической структуры кижуча Oncorhynchus kisutch (Walbaum) // Генетика. Т. 31. С. 709–714.Pustovoit S.P. Peculiarities of coho salmon Oncorhynchus kisutch (Walbaum) genetic structure. Genetics, 1995, vol. 31, № 5, pp. 709–714. (In Russian)Beacham T.D., Miller K.M., Withler R.E. 1996. Minisatellite DNA variation and stock identification of coho salmon // J. Fish Biol. Vol. 49. P. 411–429.Beacham T.D., Miller K.M., Withler R.E. 1996. Minisatellite DNA variation and stock identification of coho salmon // J. Fish Biol. Vol. 49. P. 411–429.Evanno G., Regnaut S., Goudet J. 2005. Detecting the number of clusters of individuals using the software structure: a simulation study // Mol. Ecol. Vol. 14. P. 2611–2620.Evanno G., Regnaut S., Goudet J. 2005. Detecting the number of clusters of individuals using the software structure: a simulation study // Mol. Ecol. Vol. 14. P. 2611–2620.Ivanova N.V., deWaard J., Hebert P.D.N. 2006. An inexpensive, automation-friendly protocol for recovering high-quality DNA // Mol. Ecol. Notes. Vol. 6. P. 998–1002.Ivanova N.V., deWaard J., Hebert P.D.N. 2006. An inexpensive, automation-friendly protocol for recovering high-quality DNA // Mol. Ecol. Notes. Vol. 6. P. 998–1002.Kalinowski S.T., Taper M.L., Marshall T.C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment // Mol. Ecol. Vol. 16. P. 1099–1106.Kalinowski S.T., Taper M.L., Marshall T.C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment // Mol. Ecol. Vol. 16. P. 1099–1106.Li Y.L., Liu J.X. 2018. StructureSelector: a web based software to select and visualize the optimal number of clusters using multiple methods // Mol. Ecol. Res. Vol. 18. P. 176–177.Li Y.L., Liu J.X. 2018. StructureSelector: a web based software to select and visualize the optimal number of clusters using multiple methods // Mol. Ecol. Res. Vol. 18. P. 176–177.Nakamura Y., Shigenobu Y., Sugaya T., Kurokawa T., Saitoh K. 2013. Automated screening and primer design of fi sh microsatellite DNA loci on pyrosequencing data // Ichthyological research. Vol. 60. P. 184–187.Nakamura Y., Shigenobu Y., Sugaya T., Kurokawa T., Saitoh K. 2013. Automated screening and primer design of fi sh microsatellite DNA loci on pyrosequencing data // Ichthyological research. Vol. 60. P. 184–187.Nei M. 1987. Molecular evolutionary genetics. N.Y.: Columbia Univ. press. 512 p.Nei M. 1987. Molecular evolutionary genetics. N.Y.: Columbia Univ. press. 512 p.Olsen J.B., Wilson S.L., Kretschmer E.J., Jones K.C., Seeb J.E. 2000. Characterization of 14 tetranucleotide microsatellite loci derived from sockeye salmon // Mol. Ecol. Vol. 9 (12). P. 2185–2187.Olsen J.B., Wilson S.L., Kretschmer E.J., Jones K.C., Seeb J.E. 2000. Characterization of 14 tetranucleotide microsatellite loci derived from sockeye salmon // Mol. Ecol. Vol. 9 (12). P. 2185–2187.Peakall R., Smouse P.E. 2006. GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research // Mol. Ecol. Notes. Vol. 6. P. 299–295.Peakall R., Smouse P.E. 2006. GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research // Mol. Ecol. Notes. Vol. 6. P. 299–295.Pritchard J., Stephens K., Donnely P. 2000. Inference of population structure from multilocus genotype data // Genetics. Vol. 155. P. 945–959.Pritchard J., Stephens K., Donnely P. 2000. Inference of population structure from multilocus genotype data // Genetics. Vol. 155. P. 945–959.Puechmaille S.J. 2016. The program structure does not reliably recover the correct population structure when sampling is uneven: subsampling and new estimators alleviate the problem // Mol. Ecol. Res. Vol. 16. P. 608–627.Puechmaille S.J. 2016. The program structure does not reliably recover the correct population structure when sampling is uneven: subsampling and new estimators alleviate the problem // Mol. Ecol. Res. Vol. 16. P. 608–627.Rougemont Q., Moore J.S., Leroy T., Normandeau E., Rondeau E.B., Withler R.E., Withler R.E., Van Doornik D.M., Crane P., Naish K.A., Garza J.C., Beacham T.D., Koop B.F., Bernatchez L. 2020. Demographic history shaped geographical patterns of deleterious mutation load in a broadly distributed Pacific Salmon // PLoS genetics. Vol. 16 (8). https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008348Rougemont Q., Moore J.S., Leroy T., Normandeau E., Rondeau E.B., Withler R.E., Withler R.E., Van Doornik D.M., Crane P., Naish K.A., Garza J.C., Beacham T.D., Koop B.F., Bernatchez L. 2020. Demographic history shaped geographical patterns of deleterious mutation load in a broadly distributed Pacific Salmon // PLoS genetics. Vol. 16 (8). https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1008348Smith C.T., Koop B.F., Nelson R.J. 1998. Isolation and characterization of coho salmon (Oncorhynchus kisutch) microsatellites and their use in other salmonids // Mol. Ecol. Vol. 7. P. 1614–1616.Smith C.T., Koop B.F., Nelson R.J. 1998. Isolation and characterization of coho salmon (Oncorhynchus kisutch) microsatellites and their use in other salmonids // Mol. Ecol. Vol. 7. P. 1614–1616.Smith C.T., Nelson R.J., Wood C.C., Koop B.F. 2001. Glacial biogeography of North American coho salmon (Oncorhynchus kisutch) // Mol. Ecol. Vol. 10. P. 2775–2785.Smith C.T., Nelson R.J., Wood C.C., Koop B.F. 2001. Glacial biogeography of North American coho salmon (Oncorhynchus kisutch) // Mol. Ecol. Vol. 10. P. 2775–2785.Williamson K.S., Cordes J.F., May B. 2002. Characterization of microsatellite loci in Chinook salmon (Oncorhynchus tschawytscha) and cross-species amplifi cation in other salmonids // Mol. Ecol. Notes. Vol. 2 (1). P. 17–19.Williamson K.S., Cordes J.F., May B. 2002. Characterization of microsatellite loci in Chinook salmon (Oncorhynchus tschawytscha) and cross-species amplifi cation in other salmonids // Mol. Ecol. Notes. Vol. 2 (1). P. 17–19.

The authors declare that there are no conflicts of interest present.