TAXONOMIC SIGNIFICANCE OF PHENOTYPIC AND GENOTYPIC CHARACTERS TO DESCRIBE NEW GENERA AND SPECIES
https://doi.org/10.15853/2072-8212.2018.51.60-72
Abstract
Analysis of the molecular-genetic study using old laminarialean samples collected from Russian Far East and stored in herbarium collections from 6 to 44 years is reported. To isolate algal DNA, we used two reagent kits: Invisorb Spin Plant Mini Kit (Invitek, Berlin-Buch, Germany) and Plant DNA Mini Kit (WizPrep™, Republic of Korea), and CTAB detergent (Cetyltrimethyl ammonium bromide). Rubisco, rDNA (including ITS1 and ITS2) and COI were used as DNA markers. As our data showed, after 26 years of storage of the herbarium specimens, DNA can no longer be isolated. Even in samples <26 years old, DNA can be significantly damaged, which allows to amplify only partial ITS1 and ITS2, but not Rubisco and COI. Based on comparison of genotypic and phenotypic systematics of laminarialean algae, we suggested that overreliance on genetic methods is not completely justified and approaches to describe new taxa need to be reconsidered, especially if it is impossible to collect fresh samples for the DNA extraction, and DNA is competely degraded in the material under study.
About the Authors
T. A. Klochkova
Kamchatka State Technical University
Russian Federation
Russian Federation
G. H. Kim
Kongju National University, Department of Biology
Russian Federation
Russian Federation
A. V. Klimova
Kamchatka State Technical University
Russian Federation
Russian Federation
G. .. Nina
Kamchatka State Technical University
Russian Federation
Russian Federation
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Klochkova T.A., Kim G.H., Klimova A.V., Nina G... TAXONOMIC SIGNIFICANCE OF PHENOTYPIC AND GENOTYPIC CHARACTERS TO DESCRIBE NEW GENERA AND SPECIES. The researches of the aquatic biological resources of Kamchatka and the North-West Part of the Pacific Ocean. 2018;(51):60-72. (In Russ.) https://doi.org/10.15853/2072-8212.2018.51.60-72
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